Types | DnaRegion
|
Roles | Coding
CDS
|
Sequences | BBa_K317999_sequence (Version 1)
|
Description
Pseudomonas aeruginosa produces rhamnose-containing glycolipid biosurfactants called rhamnolipids. Rhamnolipid is composed of β-D-(β-D-hydroxyalkanoyloxy)alkanoic acids(HAA) derivatized with one- or two-rhamnose sugars.In rhamnolipid biosynthesis, RhlA is required for the formation of the HAA portion of the molecule and RhlB plays a role of the rhamnosyl transferase. When RhlA and RhlB are expressed in E.coli BL21(DE3) strain, rhamno lipid indicating emulsification ability will be produced.
Notes
The coding sequence was amplified with primer sets which were designed based on 'Standard part fabrication' described in http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication
Source
Pseudomonas aeruginosa