Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matthew Orton
Date created: 2010-10-19 11:00:00
Date modified: 2015-05-08 01:11:58
yeast T1 Proximal Promoter
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K319008_sequence (Version 1) |
Description
The proximal T1 promoter was constructed with two tandem TetR operators in addition to the TATA box and start site of the GAL1 promoter. The yeast promoter is repressed by TetR (Tn10.B tetracycline repressor) and inducible with the TetR-inhibitor andhydrotetracylcine (ATc). The promoter was constructed with runs of unspecified ("N") sequence separating motifs and fixed tandem TetR operator and GAL1 motifs. The unspecified motifs modulate the efficiency of the fixed motifs to fine tune the expression of the downstream protein coding region.Notes
The part was designed using primers that introduced standard 20 bp primer binding sites flanking the upstream and downstream regions of the part.Source
The part was amplified via colony PCR from a YPH500 strain of S. cerevisiae. The strain expressing the part was given to us by Tom Ellis.Reference:
Ellis T, Wang X and Collins JJ. Diversity-based, model-guided construction of synthetic gene networks with predicted functions. Nature Biotechnology 27: 465-471 (2009).