Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Meng Lu
Date created: 2010-10-26 11:00:00
Date modified: 2015-05-08 01:11:59
CcaS upstream + PhoR downstream
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K322119_sequence (Version 1) |
Description
CcaS is a green light receptor, which has very similar downstream with the upstream of PhoR. The upstream region of CcaS senses green light, then phosphorylating the fused downstream region of PhoR. PhoR interacts with PhoB, which regulates PhoA promoter.In the presence green light, genes regulated by PhoB should be repressed.
Notes
Both proteins have a sensing domain and PAS domains in the external part of the protein, and HisKA and HATPase_c domains on the cytoplasmic side. The fusion site was chosen in the middle of the region between these outer and inner domains, by amplifying the required regions by PCR. An HolI site was added downstream of the upper CcaS region, and upstream of the lower PhoR region. HolI was chosen because the fusion scar would not introduce new amino acid, in a region that was very similar in CcaS and PhoR.Source
CcaS: Synechocystis sp. (strain PCC 6803)- Synthesised by Unam Mexico 2010.PhoR: Escherichia coli (strain K12)genomic DNA.