BBa_K322122

BBa_K322122 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K322122

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chris French and Richard Edward Partridge-Hicks

Date created: 2010-09-25 11:00:00

Date modified: 2015-05-08 01:11:59

Phycocyanobilin synthesis operon

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K322122_sequence (Version 1)

Description

Phycobilin is chromophores (light-capturing molecules) found in cyanobacteria and in the chloroplasts of red algae, glaucophytes and some cryptomonads (though not in green algae and higher plants)， used in light sensor.

Notes

Features: heme oxygenase coding sequence 63 to 629; pcyA coding sequence 650 to 1396; terminator B0015 bases 1403 to 1502 (according to the K098010 annotation, but no scar!).
Notes: the start sequence is not quite as K098010(made by Havard 2008). Basically just the first 5 bases are different from K098010, of which the first 5 bases are ctgat. Also base 24 is T rather than G. All of these are absolutely clear on the chromatogram.

Source

This is identical to K098010, deposited by Harvard 2008, except that we have transferred it to pSB1C3.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Heme oxygenase	63,629	CDS, feature/protein
Terminator (B0015)	1403,1502	feature/stop, stop_codon
pcyA	650,1396	feature/protein, CDS
J23110	1,35	promoter, feature/promoter
B0032	43,54	ribosome_entry_site, feature/rbs
rbs	630,644	ribosome_entry_site, feature/rbs

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K322122/1

Member of these Collections

• iGEM Parts Registry