BBa_K322123

BBa_K322123 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K322123

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chris French and Richard Edward Partridge-Hicks

Date created: 2010-09-24 11:00:00

Date modified: 2015-05-08 01:11:59

Phycobilin synthesis operon without terminator

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K322123_sequence (Version 1)

Description

Phycobilin is chromophores (light-capturing molecules) found in cyanobacteria and in the chloroplasts of red algae, glaucophytes and some cryptomonads (though not in green algae and higher plants)， used in light sensor.

Notes

The actual forward sequence has the same changes at the start as seen in clone H11 described above. The reverse sequence is as expected. Features same as for the one above apart from the terminator.

Source

This is derived from K098010 but PCR was used to remove the terminator, so that cph8 could be added at the end of the operon.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
J23110	1,35	promoter, feature/promoter
B0032	43,54	ribosome_entry_site, feature/rbs
Heme oxygenase	63,629	CDS, feature/protein
rbs	630,644	feature/rbs, ribosome_entry_site
pcyA	650,1396	CDS, feature/protein

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K322123/1

Member of these Collections

• iGEM 2018 Distribution Plate 6
• iGEM 2018 Distribution Plate 6 Well 5M
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM 2019 Distribution Plate 4
• iGEM 2019 Distribution Plate 4 Well 5M
• iGEM 2019 Part Library
• iGEM 2019 Plasmid pSB1C3
• iGEM Parts Registry