BBa_K322211

BBa_K322211 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K322211

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: William Rostain and Meng Lu

Date created: 2010-09-23 11:00:00

Date modified: 2015-05-08 01:11:59

Red emitting firefly luciferase mutant (356K)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K322211_sequence (Version 1)

Description

This is the same as BioBrick [9; add proper code when assigned] but has lysine inserted between amino acids 355 and 356 of the native sequence. This has been reported to result in a red-shifted output.

Features: introduced lysine codon 1066 to 1068

reference:

Notes

Sequence date: the mutation was initially introduced into our construct K216015 and the sequence across the mutation was obtained on 12 Aug 2010 (clone 4). The coding sequence was then amplified by PCR and cloned in pSB1C3 by Will and Lu.

Source

Photinus pyralis

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Introduced lysine	1066,1068	sequence_alteration, feature/mutation

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K322211/1

Member of these Collections

• iGEM Parts Registry