BBa_K322237

BBa_K322237 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K322237

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chris French and William Galdric Rostain

Date created: 2010-09-25 11:00:00

Date modified: 2016-01-28 12:23:44

Firefly luciferase from Photinus pyralis

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K322237_sequence (Version 1)

Description

In luminescent reactions, light is produced by the oxidation of a luciferin (a pigment):
luciferin + O2 → oxyluciferin + light

Thus luciferase is a a reporter protein when connected to other proteins or promoters prodicing light, by the oxidation of substrate luciferin.


This is derived from BioBrick I712019, but PCR was used to remove the extra bases before the start codon (these are shown in the Registry sequencing data but are not indicated in the sequence as entered by Ljubljana 2007; presumably they are there to make a proper eukaryotic context for the start codon). This BioBrick contains the bare coding sequence in pSB1C3.

Notes

Forward sequence looks good. Reverse sequence missing the base between the stop codon and SpeI site. This is also missing in the S284T clone (see below) suggesting that it is missing in the primer, or did we use a vector based primer? In which case it would suggest that it was missing in the original clone. Trivial but annoying.

Source

The origional sequence is from Photinus pyralis, a the gene here is derived from BioBrick BBa-I712019.

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K322237/1

Member of these Collections

• //cds/reporter
• //function/reporter/light
• iGEM Parts Registry