BBa_K324000

BBa_K324000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K324000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Julius Ho

Date created: 2010-10-24 11:00:00

Date modified: 2015-05-08 01:12:02

LacI repressor protein in RFC25 format

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K324000_sequence (Version 1)

Description

This part consists of the lacI repressor protein (BBa_I732100) optimized with endings for protein fusion in the Freiburg assembly format (RFC25). There is also a 6-his tag appended to the C-terminus for easy purification using Ni-affinity column. RFC 25 prefix/suffix and 6-his sequences attached via PCR.

Notes

6-his tag was appended to the 3' end of this protein to allow for purification upon expression in E. coli. We chose a C-terminal tag to avoid isolation of incompletely translated peptides.

Source

http://partsregistry.org/Part:BBa_I732100
Submitted by the USTC '07 iGEM team, taken from the 2010 Spring Distribution

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
LacI in RFC25	1,1104	CDS, feature/protein
double stop codon	1098,1104	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K324000/1

Member of these Collections

• iGEM Parts Registry