Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julius Ho
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:12:02
Tat protein transduction domain with glycine linker (RFC25)
| Types | DnaRegion |
| Roles | polypeptide_domain Protein_Domain |
| Sequences | BBa_K324002_sequence (Version 1) |
Description
This part consists of the Tat protein transduction domain, an 11 a.a. sequence able to carry attached proteins across cell membranes without need to permeabilize. This domain, attached to a glycine linker, can be joined to other peptides in the RFC25 Freiburg assembly format to create fusion proteins that can freely transduce cell membranes. Our part consists of the Tat-glycine linker, intended to be attached to the N-terminus of any fusion protein construct.The original Tat a.a. sequence comes from the Tat protein in human immunodeficiency virus but does not possess any innately pathogenic features. Bsl2 safety precautions are generally recommended for Tat-fusion proteins, although Tat DNA can be manipulated without special protocols.
Notes
The RFC25 fusion assembly format was used because this protein domain is intended to be fused to other proteins to convey its membrane-crossing characteristics. N terminal and/or C terminal addition of the Tat domain have both been shown to be effective.Source
part synthesized from the seequence of the Tat protein in human immunodeficiency virus| Sequence Annotation | Location | Component / Role(s) |
| Start codon glycine Tat domain glycine linker | 1,3 4,6 7,39 40,63 | start_codon feature/start feature/protein CDS feature/protein CDS CDS feature/protein |