BBa_K324004

BBa_K324004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K324004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Julius Ho

Date created: 2010-10-24 11:00:00

Date modified: 2015-05-08 01:12:02

AraC inducible ChFP generator

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K324004_sequence (Version 1)

Description

This part combines two previously available BioBricks via standard assembly: a promoter inducible by AraC (BBa_R0080) and CherryFP generator (BBa_J06702). This composite acts a reporter in E. coli, allowing cells to produce cherry fluorescent protein in the presence of AraC protein. In the absence of AraC, minimal levels of fluorescence should be observed.

The cherry FP generator consists of an RBS, mCherry, and double terminator.

Notes

n/a

Source

Both of this composite's constituent parts, the AraC inducible promoter (BBa_R0080) and CherryFP generator (BBa_J06702), are available from the Spring 2010 Distribution.

Components

Access	Instance	Definition
public	BBa_R0080	AraC
public	BBa_J06702	mCherry

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0080	1,149	AraC
BBa_J06702	158,1026	mCherry

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K324004/1

Member of these Collections

• iGEM Parts Registry