BBa_K327018

BBa_K327018 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K327018

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Keegan Owsley

Date created: 2010-10-24 11:00:00

Date modified: 2015-05-08 01:12:03

LVA-tagged cI repressor from phage lambda

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K327018_sequence (Version 1)

Description

Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).

This part is identical in design to BBa_C0051, but the barcode has been removed. The 2010 UC Davis team found that this part, in combination with the barcode, produced an endemic constitutive promoter, which initiated transcription of any coding regions placed downstream. This part, which lacks the barcode, does not exhibit this behavior.

Notes

Removed barcode from coding region.

Source

From registry

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
cI lambda	1,711	feature/BioBrick, engineered_region
LVA	712,744	tag, feature/tag

Other Properties

• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K327018/1

Member of these Collections

• iGEM Parts Registry