BBa_K331021

BBa_K331021 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K331021

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Adam Smith

Date created: 2010-09-25 11:00:00

Date modified: 2015-05-08 01:12:04

Catechol 2,3 Dioxygenase with Fusion Suffix

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K331021_sequence (Version 1)

Description

This is the product of the xylE gene from Pseudomonas putida. The suffix of this part has been altered to the Silver lab standard for fusion proteins.


This part is ready for the addition of a protein at its N-terminus to create fusion protein

Notes

We chose to remove the promoter and endogenous ribosomal binding site (of J33204) so we could add a promoter of our choosing

Source

This part is the PCR product of BBa_K118021 using the following primers:

Forward: 5'-GAA TTC GCG GCC GCT TCT AGA TGA ACA AAG GTG TAA TGC GAC CG-3' (xylE Prefix Standard)

Reverse: 5'-CTG CAG CGG CCG CTA CTA GTG GTC AGC ACG GTC ATG AAT CG-3' (xylE Suffix Fusion)

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• rdf-schema#seeAlso: P06622
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K331021/1

Member of these Collections

• iGEM Parts Registry