Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sean Kearney
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:12:05
ODD with Luciferase
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K334002_sequence (Version 1) |
Description
Part of the degradation domain taken from the HIF-1a protein in humans. Under normal oxygen conditions, prolyl hydroxylases target this domain for hydroxylation. The hydroxylated subunit makes the resultant protein a target for E3 ubiquin ligase when bound by the VHL gene product, leading to its proteasomal degradation. When fused to the luciferase, and following a strong, mammalian constitutive promoter, it should indicate low levels of oxygen in cell populations.Notes
The part has to mimic the activity of HIF-1a in mammalian systems by degrading at normal oxygen concentrations and upregulating at high oxygen concentrations. The fusion of the degradation domain to a reporter theoretically enables this activity.Source
It comes from the HIF1A gene in homo sapiens sapiens.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K334000 BBa_I712019 | 1,177 178,1827 | engineered_region feature/BioBrick engineered_region feature/BioBrick |