BBa_K337016

BBa_K337016 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K337016
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R
Date created: 2010-10-20 11:00:00
Date modified: 2015-05-08 01:12:06

synthetic microRNA HD10



Types
DnaRegion

Roles
mature_transcript_region

RNA

Sequences BBa_K337016_sequence (Version 1)

Description

Synthetic microRNA HD10 is a adaption of microRNA hsa-mir122 to a synthetic target sequence. This microRNA is oriented reverse-complementary and can be driven with any Pol2 promoter of the users choice.

Notes

Guiding and passanger strand of the microRNA HD10 were constructed to have no endogenous human target. Adaption to microRNA hsa-mir122 was done for having a proper expression frame and enabling expression the microRNAs from Pol2 promoters.

Source

synthetic

Sequence Annotation Location Component / Role(s)
passanger strand
loop
guiding strand
54,75
76,87
88,109
feature/misc sequence_feature
feature/misc sequence_feature
feature/misc sequence_feature
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K337016/1