BBa_K337020

BBa_K337020 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K337020

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R

Date created: 2010-10-20 11:00:00

Date modified: 2015-05-08 01:12:06

BGH(rc)/shRNA10(rc)/sv40(rc)/RSV(fw)

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K337020_sequence (Version 1)

Description

SV40 promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator. Those elements are all rc, RSV promoter near SV40 drives in the opposite direction (fw).

Notes

standard assembly

Source

ggatccccatagagcccaccgcatccccagcatgcctgctattgtcttcccaatcctcccccttgctgtcctgccccaccccaccccccagaatagaatgacacctactcagacaatgcgatgcaatttcctcattttattaggaaaggacagtgggagtggcaccttccagggtcaaggaaggcacgggggaggggcaaacaacagatggctggcaactagaaggcacagtcgaggctgatcagcgggtttaaaGCTAGTAAGCAAACGATGCCAAGACATTTATCGAGGGAAGGATTGCCTAGCAGTAGCTAGGTACCACGGTCACCGATATTAATAGTTTAGACATAATATCGGTGGCCGTGGTGTCCAGCTCTGCTAAGGAAACTCTGTAGCCACGAAGGTGTTAACTTCACCTCCAGCTAGTaagctttttgcaaaagcctaggcctccaaaaaagcctcctcactacttctggaatagctcagaggccgaggcggcctcggcctctgcataaataaaaaaaattagtcagccatggggcggagaatgggcggaactgggcggagttaggggcgggatgggcggagttaggggcgggactatggttgctgactaattgagatgcatgctttgcatacttctgcctgctggggagcctggggactttccacacctggttgctgactaattgagatgcatgctttgcatacttctgcctgctggggagcctggggactttccacaccctaactgacacacattccacagctggttctttccgcctcagaaggtacctaaccaagttcctctttcagaggttatttcaggccatggtgctgcgcGCTAGTCAATTCAtgTTTGACAGCTTATCATCGCAGaTCCGTATGGTGCACTCTCagtACAATCTGCTctgATGCCGCATaGtTAAGCCAGTATCTGCTCCctgCttgTGTGTTGGagGTCGctgagtAGTGCGCgaGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTATCTGAGGGGACTAGGGTGTGTTTAGGCGAAAAGCGGGGCTTCGGTTGTACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACTGAATACCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGG

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
synthetic microRNA part BBa_K337016	261,421	sequence_feature, feature/misc
BGH Terminator	1,255	sequence_feature, feature/misc
SV40	427,846	feature/promoter, promoter
RSV	851,1471	promoter, feature/promoter

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K337020/1

Member of these Collections

• iGEM Parts Registry