BBa_K337030

BBa_K337030 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K337030
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R
Date created: 2010-10-21 11:00:00
Date modified: 2015-05-08 01:12:06

Luc2_SV40 (microRNA binding site compatible)



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Reporter

    Sequences BBa_K337030_sequence (Version 1)

    Description

    Luc2 with XmaI/XhoI site inserted in the 3'UTR right behind the stop codon (BB-2, RFC12).

    Notes

    XmaI/XhoI sites were integrated into the 3'UTR to enable easy insertion of microRNA binding sites right behind the coding sequence.

    Source

    pGl4.23

    Sequence Annotation Location Component / Role(s)
    Luc2
    XmaI
    XhoI
    SV40 Terminator
    		1,1659
    1670,1670
    1686,1686
    1695,1949
    		CDS feature/cds
    feature/misc sequence_feature
    feature/misc sequence_feature
    feature/misc sequence_feature

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#topLevel
    BBa_K337030/1
     

    Attachments

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