Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rebecca Berrens
Date created: 2010-10-25 11:00:00
Date modified: 2015-05-08 01:12:06
synthetic binding site of shRNA 122 (perfect) KD96:%
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K337055_sequence (Version 1) |
Description
perfect binding site of liver specific shRNA 122 in the context of the tuning construct. This binding side leads to a knockdown percentage of 96% percent if introduced into the 3'UTR of the gene of interest (GOI). It can be either co-transfected with a synthetic miRNA or transfected into liver cell lines (i.e. Huh7).Notes
designed to lead to 100% KD if introduced into the 3'UTR of your GOI and transfected into liver cell lines or co-transfected with miR122Source
synthetic| Sequence Annotation | Location | Component / Role(s) |
| seed miR122 | 15,22 | feature/misc sequence_feature |