BBa_K338062

BBa_K338062 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K338062

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lucas Hartsough

Date created: 2010-10-26 11:00:00

Date modified: 2015-05-08 01:12:07

GFP Reporter Device, Heat-Shock or IPTG Induced

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K338062_sequence (Version 1)

Description

Construct assembled to allow the quantitative analysis of the heat shock/LacI promoter/repressor system, BBa_K338002. Produces GFP in response to low-temperature heat shock, more-so in the presence of LacI. GFP production is retarded in LacIq cells (or in the presence of +5mM glucose) about 4-6x. See K338002 for more detailed data.

Notes

The type of GFP used in this construct happens to have a very long half life. If growing cultures for fluorescence measurements, grow at 30??C - any higher will activate the promoter, causing GFP to be produced.

Source

The Registry of Standard Biological Parts

Components

Access	Instance	Definition
public	BBa_K338002	HSP-LacI
public	BBa_B0034	BBa_B0034
public	BBa_E0040	GFP
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K338002	1,181	HSP-LacI
BBa_B0034	190,201	BBa_B0034
BBa_E0040	208,927	GFP
BBa_B0015	936,1064	BBa_B0015

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K338062/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //direction/forward
• //promoter
• //regulation/positive
• //rnap/prokaryote/ecoli/sigma32
• iGEM Parts Registry