Recombination sequence 1 and 2, which is homologous to part of modified E.coli genome, the CDS of gene chlr and I-Sec I cutting site are all included in the MCS of PSB1A13 plasmid. The biorick is designed to verify the "landing pad method" we use in our project.
Notes
As the biobrick we use contains gene Chlr, we have to choose PSB1A13 as the backbone of our parts.