Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Teng Li
Date created: 2010-10-20 11:00:00
Date modified: 2015-05-08 01:12:08
Chloramphenicol resistance(with Promoter) + I-sceI site+ Recombination site
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K341458_sequence (Version 1) |
Description
Chloramphenicol resistance gene(with Promoter) + I-sceI site+ Recombination site+DraIII cutting site. This part can be used as a recombination unit between a plasmid and the chromosome in the "In-vivo Recombination System" in iGEM project "Tsinghua 2010"Notes
This part involves DraIII cutting site at two ends of the unit, this cutting site is compatible with BBF RFC 61Source
Chloramphenicol resistance gene is form plasmid "pKD3"| Sequence Annotation | Location | Component / Role(s) |
| Cm Resistance gene recombination sequence Recombination sequence DraIII cutting site DraIII cutting site I-secI cutting site I-secI cutting site | 111,749 33,57 953,977 6,14 996,1004 15,32 978,995 | feature/cds CDS sequence_feature feature/dna sequence_feature feature/dna sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature |