Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ceren Seref
Date created: 2010-10-01 11:00:00
Date modified: 2015-05-08 01:12:11
pCooM Promoter from Rhodospirillum rubrum
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K352003_sequence (Version 1) |
Description
The two CooA-regulated R. rubrum promoters, PcooF and PcooM, contain 2-fold symmetric DNA sequences that serve as CooA-binding sites and are similar to the CRP consensus sequence. This is consistent with the similarity between CooA and CRP in their helix-turn-helix motifs. The CooA binding sites lie at the 243.5 and 238.5 positions relative to the transcription start sites in PcooF and PcooM, respectively, overlapping with the 235 region. This overlap suggests that both CooA-regulated promoters are analogous to class II CRP-dependent promoters. We chose PcooF for this study because it is the stronger promoter based on the amount of primer extension product and level of coo-encoded proteins synthesized in vivo.The CO-dependent anaerobic growth of Rhodospirillum rubrum relies on a CO oxidation system encoded by two CO regulated transcriptional units, cooMKLXUH and cooFSCTJ. The key products of the coo regulon are an O2- sensitive CO dehydrogenase (CooS), a CooS-associated Fe-S protein (CooF), and a CO-tolerant hydrogenase (CooH), and the expression of the genes depends upon the activity of the CooA protein, which senses CO under anaerobic conditions.
Notes
The sequence information was acquired from NCBI and physical DNA was synthesized from GENEART. Classical cloning strategies(restriction digestion) were used to produce biobricks.Source
Y. He, T. Gaal, R. Karls, T. Donohue, R. Gourse and G.P. Roberts, J. Biol. Chem. 274 (1999), pp. 10840???10846.| Sequence Annotation | Location | Component / Role(s) |
| CooA binding Site CooA binding Site | 14,18 25,29 | feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding |