BBa_K353002

BBa_K353002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K353002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alejandro Virrueta

Date created: 2010-10-24 11:00:00

Date modified: 2015-05-08 01:12:11

I0500 + K353010 + B0034 + E0040 + B1006

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K353002_sequence (Version 1)

Description

This part is composed of an inducible arabinose promoter (I0500), an RNA tag, a ribosome binding site (B0034), GFP (E0040), and a terminator (B1006). This is one of the four subsystems utilized in detecting a ratio (see Stanford iGEM 2010 project). Arabinose induces the production of an mRNA transcript, and if no sRNA1 is present (see part ), this can then be translated into GFP.

Notes

We designed the RSID1 tag. It was developed as to not interfere with E. coli's native genome and to be bound by sRNA1 (see part ).

Source

This part is assembled from other parts found in the registry (except for the RSID tag).

Components

Access	Instance	Definition
public	BBa_I0500	pBad/araC
public	BBa_K353010	BBa_K353010
public	BBa_E0040	GFP
public	BBa_B1006	BBa_B1006

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I0500	1,1210	pBad/araC
BBa_K353010	1219,1303	BBa_K353010
BBa_E0040	1310,2029	GFP
BBa_B1006	2038,2076	BBa_B1006

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K353002/1

Member of these Collections

• iGEM Parts Registry