Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michelle Robinson
Date created: 2010-10-18 11:00:00
Date modified: 2015-05-08 01:12:11
PlcR-PapR - Gram+ve Quorum Peptide
| Types | DnaRegion |
| Roles | engineered_region Signalling |
| Sequences | BBa_K354001_sequence (Version 1) |
Description
The PlcR-PapR fusion peptide has been derived from the Bacillus cereus quorum signalling system. This group of Gram-positive bacteria employs the PlcR quorum system to initiate virulence factors under appropriate cell density conditions. Synthesized as a propeptide, this fusion molecule is exported from the cell via the secA pathway, cleaved by an extracellular protease and re-imported into the cell via an oligopeptide permease. Both the extracellular protease as well as olidopeptide permease are ubiquitous structures to the Gram-positive cell wall. A such, any gram-positive bacteria expressing the PlcR-PapR protein should be able to both process as well as import the activating form of the fusion peptide.Notes
The sequence recognised by the SecA pathway had to be included in the synthesised sequence so as to ensure that the fusion peptide is adequately exported fro the cell. A ribozyme binding site (RBS) was also included in the final sequence. Furthermore, the sequence was codon optimized for Lactobacillus gasseri.Source
This sequence was adapted from the naturally-occurring fusion peptide found in the Bacillus cereus genome.| Sequence Annotation | Location | Component / Role(s) |
| Shine Dalgarno seq Start codon | 6,12 20,23 | ribosome_entry_site feature/rbs feature/start start_codon |