Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ken Kajita
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:12:12
Lysisbox -type2- ver2
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K358024_sequence (Version 1) |
Description
This part is the same to BBa_K358021[Lysisbox -module2-], except for its constitutive promoter[BBa_J23101].So, this part also induces constitutively SΔTMD1 gene, the anti-killer gene, and lambda lysis cassette[SRRz], the killer gene, is regulated by lacP.
Since the lysis cassette is toxic to E.coli, you must repress the lacP when you transform it. Because of this, Top 10 isn't suitable, we used KRX.
This is a long part and a lactose operon was sometimes deleted unexpectedly in our experiments. So, we find that to set the cultural temperature at 30C and store this part as a plasmid, not a glycerol stock, is the prefareble.
Notes
We used PCR to change the constitutive promoter.Source
BBa_K358021| Access | Instance | Definition |
| public public public | BBa_J23101 BBa_K358010 BBa_K358019 | BBa_J23101 BBa_K358010 BBa_K358019 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23101 BBa_K358010 BBa_K358019 | 1,35 44,1218 1227,2699 | BBa_J23101 BBa_K358010 BBa_K358019 |