Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jacky, Fong Chuen, Loo
Date created: 2010-10-23 11:00:00
Date modified: 2015-05-08 01:12:12
Rci site-specific recombinase
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K361000_sequence (Version 1) |
Description
This part will prouce a 384-amino acid Rci site-specific recombinase used in shufflon system, which is a site-specific DNA recombination between two specific repeat sites as recognition.After this recombinase is being expressed, it can alter the DNA sequence being inserted randomly with the specific repeat sites originally to become an unknown sequence.
Notes
The gene is come from muliple inversion systems in R64 plasmid and there is no further modification.Reports have shown that some modification of the gene will lead to deletion some parts of DNA sequence during recombination process.
Source
It belongs to integrase family of site-specific recombinase, and it is extracted from muliple inversion systems in R64 plasmid, found in genomic sequence code:AP005147.1| Sequence Annotation | Location | Component / Role(s) |
| Rci recombinase | 1,1155 | CDS feature/protein |