Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Noah Young
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:12:13
A characterized calcium dependent response element binding site for the Crz1 activator
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K363006_sequence (Version 1) |
Description
This part is a binding site for the Crz1 transcriptional activator. When placed upstream of a eukaryotic promoter sequence, this sequence results in the calcium dependant transcription of downstream coding sequences given that Crz1 and the upstream signal transducing phosphotase calcineurin are constitutively expressed. The signal cascade occurs when calcineurin binds to cytosolic calcium, activating its phosphotase activity. The active calcineurin dephosphorylates cytoplasmic Crz1, resulting in translocation of Crz1 to the nucleus where it binds to this part and promotes the assembly of general transcription factors at the promoter sequence.Notes
This part is NOT itself a promoter. It must be inserted upstream of a promoter to function.Source
This part was derived from calcium dependent response element (CDRE) binding sequence of the FSK2 gene. The consensus sequence for Crz1 binding as described by Yoshimoto 2002 was mutated at non conserved residues to generate this part. This sequence differs by only one base pair from the consensus. The second base, A in the consensus, is a T in this sequence. We estimate it occurs as a Crz1 binding site with ~1/4 the frequency of the consensus.| Sequence Annotation | Location | Component / Role(s) |
| Crz1 Binding Site | 1,7 | feature/binding non_covalent_binding_site |