BBa_K363006

BBa_K363006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K363006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Noah Young
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:12:13

A characterized calcium dependent response element binding site for the Crz1 activator



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K363006_sequence (Version 1)

Description

This part is a binding site for the Crz1 transcriptional activator. When placed upstream of a eukaryotic promoter sequence, this sequence results in the calcium dependant transcription of downstream coding sequences given that Crz1 and the upstream signal transducing phosphotase calcineurin are constitutively expressed. The signal cascade occurs when calcineurin binds to cytosolic calcium, activating its phosphotase activity. The active calcineurin dephosphorylates cytoplasmic Crz1, resulting in translocation of Crz1 to the nucleus where it binds to this part and promotes the assembly of general transcription factors at the promoter sequence.

Notes

This part is NOT itself a promoter. It must be inserted upstream of a promoter to function.

Source

This part was derived from calcium dependent response element (CDRE) binding sequence of the FSK2 gene. The consensus sequence for Crz1 binding as described by Yoshimoto 2002 was mutated at non conserved residues to generate this part. This sequence differs by only one base pair from the consensus. The second base, A in the consensus, is a T in this sequence. We estimate it occurs as a Crz1 binding site with ~1/4 the frequency of the consensus.

Sequence Annotation Location Component / Role(s)
Crz1 Binding Site
1,7
feature/binding non_covalent_binding_site
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BBa_K363006/1