BBa_K364304

BBa_K364304 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K364304

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Endre K??roly Krist??f

Date created: 2010-08-12 11:00:00

Date modified: 2015-05-08 01:12:14

TRE-CMV

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K364304_sequence (Version 1)

Description

The Tetracycline Response Element (TRE) is recognized and bound by the Tetracycline repressor (TetR) protein. The TRE consists of 7 repeats separated by spacer sequences and placed upstream of CMV minimal promoter that has basal expession in the abscence of bound TetR. Tetracycline derivatives (e.g. doxycycline) bind TetR and render it incapable of binding to TRE, thereby forcing the expression of target genes.

Notes

Compatible with RFC-25 and RFC-10.

Source

Arteficial

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
TRE	1,249	feature/operator, operator
minimal P-CMV	255,315	promoter, feature/promoter
1st tetO	9,27	operator, feature/operator
2nd tetO	45,63	operator, feature/operator
3rd tetO	80,98	operator, feature/operator
4th tetO	116,134	operator, feature/operator
5th tetO	152,170	operator, feature/operator
6th tetO	187,205	operator, feature/operator
7th tetO	223,241	feature/operator, operator

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K364304/1

Member of these Collections

• iGEM Parts Registry