PIF-3

BBa_K365000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K365000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: LACOTTE Yohann
Date created: 2010-09-13 11:00:00
Date modified: 2015-05-08 01:12:15

Phytochrome Interacting Factor-3 (aa 1-100)



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K365000_sequence (Version 1)

Description

First hundred aminoacids of Phytochrome Interacting Factor 3 (PIF3) from A. thaliana, which physically interacts with Phytochrome B.

Notes

We needed to delete one restriction site by directed mutagenesis.

Source

The sequence of full lenght PIF-3 cDNA was a gift from Dr Kircher (Universit??t Freiburg).

Sequence Annotation Location Component / Role(s)
start
T > A
1,3
177,177
feature/start start_codon
feature/s_mutation point_mutation
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K365000/1