PhyB-908-(

BBa_K365011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K365011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: RENAULT Renaud

Date created: 2010-09-14 11:00:00

Date modified: 2015-05-08 01:12:15

Phytochrome B (aa 1-908) linked to a trimer of ClpX (aa 61-425)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K365011_sequence (Version 1)

Description

Phytochrome B (aa 1-908) was fused to a trimer of ClpX in order to tether substrate containing PIF to the protease upon light application, thus promoting its degradation.

Notes

We used Freiburg fusion standard (RFC-25) for in frame assembly.

Source

See subparts.

Components

Access	Instance	Definition
public	BBa_K365002	PhyB-900
public	BBa_B0105	Scar 25
public	BBa_K365005	linker
public	BBa_B0105	Scar 25
public	BBa_K365004	ClpX
public	BBa_B0105	Scar 25
public	BBa_K365005	linker
public	BBa_B0105	Scar 25
public	BBa_K365004	ClpX
public	BBa_B0105	Scar 25
public	BBa_K365005	linker
public	BBa_B0105	Scar 25
public	BBa_K365004	ClpX

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K365002	1,2724	PhyB-900
BBa_B0105	2725,2730	Scar 25
BBa_K365005	2731,2790	linker
BBa_B0105	2791,2796	Scar 25
BBa_K365004	2797,3888	ClpX
BBa_B0105	3889,3894	Scar 25
BBa_K365005	3895,3954	linker
BBa_B0105	3955,3960	Scar 25
BBa_K365004	3961,5052	ClpX
BBa_B0105	5053,5058	Scar 25
BBa_K365005	5059,5118	linker
BBa_B0105	5119,5124	Scar 25
BBa_K365004	5125,6216	ClpX

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K365011/1

Member of these Collections

• iGEM Parts Registry