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BBa_K365013

BBa_K365013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K365013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: RENAULT Renaud

Date created: 2010-10-09 11:00:00

Date modified: 2015-05-08 01:12:15

ClpX trimer

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Details

Types	DnaRegion
Roles	Coding CDS
Sequences	BBa_K365013_sequence (Version 1)

Description

We fused 3 ∆N-ClpX with 20 aa linker in order to increase the stability of ClpX pseudo-hexamer.

Notes

See subparts.

Source

∆N-ClpX was initially extracted from E. coli genome using colony PCR, then we deleted some restriction sites. We then used standard assembly protocols.

Components

Access	Instance	Definition
public public public public public public public public public	BBa_K365004 BBa_B0105 BBa_K365005 BBa_B0105 BBa_K365004 BBa_B0105 BBa_K365005 BBa_B0105 BBa_K365004	ClpX Scar 25 linker Scar 25 ClpX Scar 25 linker Scar 25 ClpX

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K365004 BBa_B0105 BBa_K365005 BBa_B0105 BBa_K365004 BBa_B0105 BBa_K365005 BBa_B0105 BBa_K365004	1,1092 1093,1098 1099,1158 1159,1164 1165,2256 2257,2262 2263,2322 2323,2328 2329,3420	ClpX Scar 25 linker Scar 25 ClpX Scar 25 linker Scar 25 ClpX

Other Properties

igem#experience	None
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K365013/1