| Types | DnaRegion
|
| Roles | Composite
engineered_region
|
| Sequences | BBa_K367012_sequence (Version 1)
|
Description
Gene???s fecI and fecR of Escherichia coli, encoding an ECF sigma factor and a membrane signal transduction protein respectively, involved in signal transduction of the ferric citrate-dependent Fec system. They have been synthesized in the same Biobrick because of their 4 bp overlap in the E. coli genome, not to reduce their expression. FecR protein interacts with the outer membrane sensor FecA and activates the sigma factor FecI, which acts over PfecA promoter region.
Notes
Stop codon substituted by standard biobrick TAATAA double stop. PstI site at 1184 removed: CTGCAG to CTGCAA.
Source
This part has been made by PCR and SDM using E. coli K12 genome as template.
