Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Emanuel Stiegeler
Date created: 2010-07-30 11:00:00
Date modified: 2015-05-08 01:12:15
Pbad::pduD-aurF
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K368000_sequence (Version 1) |
Description
This part consists of two units. First is an inducible promoter (BBa_I0500). The second is a translational fusion of two proteins (pduD-aurF). PduD is a subunit of a diol dehydratase, which is located in the bacterial microcompartment (BMC) of Citrobacter freundii (Parsons et al, 2010). AurF is a para-aminobenzoate N-oxygenase from Streptomyces thioluteus (Simurdiak et al., 2006). When pduD was amplified a consensus RBS (AGGAGG)were introduced by using a corresponding primer (GCA GAATTC GCGGCCGC T TCTAGA AGGAGG GTAGTC ATGGAAATCAATGAAAAGC). The two sequences were fused by PCR. For this purpose aurF were amplified with a 5' overhang, which is identical to the last 25 bases of pduD. To obtain a translational fusion this overhang-sequence doesn??t contain the stop codon of pduD.Notes
This part is under construction. As soon as necessary informations and problems will be communicated.Source
pduD was amplified from gDNA of C.freundii (DSMZ strain number: DSM 30039)aurF was amplified from gDNA of S. thioluteus (DSMZ strain number: 40027)
| Sequence Annotation | Location | Component / Role(s) |
| AraC PC Pbad BBa_I0500 SpeI-XbaI scar consensus rbs incl. spacer (2 Valins) pduD with removed stop codon aurF | 1,1079 1043,1114 1080,1210 1,1210 1211,1216 1217,1228 1229,1900 1901,2914 | sequence_feature feature/misc promoter feature/promoter promoter feature/promoter feature/BioBrick engineered_region sequence_feature feature/dna feature/rbs ribosome_entry_site CDS feature/protein CDS feature/protein |