BBa_K368007

BBa_K368007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K368007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jara Radeck
Date created: 2010-10-23 11:00:00
Date modified: 2015-05-08 01:12:15

tet-on Promoter (prev TRE) + TEV recogn. site+ N-degron+SF3b155



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K368007_sequence (Version 1)

    Description

    eukaryots:
    tet-on Promoter (prev TRE): inducible CMV promoter, inducible by doxycycline
    TEV recogn. site: specific recognition site for TEV protease
    N-degron: Protein degradation signal, if N-terminus is free
    SF3b155: Splicing factor 3b, size: 155kDa; interacting with p14*

    Notes

    -> "Cut'N'Survive" system

    Source

    (Knop et al.: Efficient protein depletion by genetically controlled deprotection of a dormant N-degron (2009))

    Access Instance Definition
    public
    public
    		BBa_K368001
    BBa_K368019
    		BBa_K368001
    BBa_K368019

    Sequence Annotation Location Component / Role(s)
    BBa_K368001
    BBa_K368019
    		1,444
    451,1635
    		BBa_K368001
    BBa_K368019

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K368007/1
     

    Attachments

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