Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yang Zhang
Date created: 2010-08-26 11:00:00
Date modified: 2015-05-08 01:12:15
pduAB(Propanediol utilization gene A+B)[RBS+pduA+RBS+pduB]
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K371001_sequence (Version 1) |
Description
Pdu, a abbreviation of propanediol utilization, is name of operon which is used for degradation propanediol to generate energy. Pdu operon consist of 21 genes. Some of these genes encode a series proteins which assemble to a large icosahedral protein complex.PduAB consist of pdu A and pdu B wich their corresponding natural RBS, which got from Citrobacter Freundii by PCR. PduAB, together with others struture part of pdu operon such as pdu JK, can be used to construct a empty proteinous microcompartment in bacteria.
Notes
The sequencing result show that the gene of pduAB has some silent mutations, which have no effect on the function of protein.Source
pduJK come from Citrobacter Freundii genenom(NBRC NO.12681)| Sequence Annotation | Location | Component / Role(s) |
| RBS pduA coding sequence start codon stop codon pduB coding sequence RBS stop codon start codon | 6,11 19,300 300,302 298,300 300,1112 285,292 1110,1112 19,21 | ribosome_entry_site feature/rbs CDS feature/cds feature/start start_codon stop_codon feature/stop CDS feature/cds feature/rbs ribosome_entry_site stop_codon feature/stop start_codon feature/start |