Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yang Zhang
Date created: 2010-08-26 11:00:00
Date modified: 2015-05-08 01:12:16
pduTUV(Propanediol utilization gene T+U+V)[RBS+pduT+RBS+pduU+RBS+pduV]
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K371005_sequence (Version 1) |
Description
Pdu, a abbreviation of propanediol utilization, is name of operon which is used for degradation propanediol to generate energy. Pdu operon consist of 21 genes, which belongs to a kind of BMC(bacterial microcompartment).Some of these genes encode a series proteins which assemble to a large icosahedral protein complex.PduTUV consist of pduT, pduU and pduV wich their corresponding natural RBS, which got from Citrobacter Freundii by PCR. PduTUV, together with other struture parts of pdu operon, can be used to construct a empty proteinous microcompartment in bacteria.
Notes
Sequencing result shows that there are several silent mutation in pduT and pduU. PduV has some error mutation. We are still working on the function assay to certify whether the mutation pduV still function well.Source
pduJK come from Citrobacter Freundii genenom(NBRC NO.12681)| Sequence Annotation | Location | Component / Role(s) |
| stop codon pduU coding sequence pduV coding sequence RBS RBS RBS start codon start codon start codon stop codon stop codon pduT coding sequence | 1393,1396 589,939 944,1396 23,28 576,582 931,937 35,47 589,591 944,946 587,589 937,939 35,589 | stop_codon feature/stop feature/cds CDS feature/cds CDS feature/rbs ribosome_entry_site feature/rbs ribosome_entry_site feature/rbs ribosome_entry_site feature/start start_codon start_codon feature/start start_codon feature/start stop_codon feature/stop stop_codon feature/stop feature/cds CDS |