BBa_K380004

BBa_K380004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K380004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Johan Nordholm, Andreas Constantinou, Nina Schiller

Date created: 2010-10-24 11:00:00

Date modified: 2015-05-08 01:12:17

Tp10 cell-penetrating peptide

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K380004_sequence (Version 1)

Description

Transportan 10 (Tp10) is a cell-penetrating that may be used in N-terminal fusions with full-length proteins to create transduction proteins with the ability to permeate the lipid bilayer of various cell types, making it a potential gene or protein delivery vector.

Chemically synthesized Tp10 peptides conjugated to different cargo, including pDNA and protein, have been shown to efficiently penetrate the lipid bilayer of both human and mouse cells (Kilk et al., 2005). Membrane permeation is both energy and temperature independent (Hällbrink et al., 2001). The exact mechanism for penetration is still unclear (Yandek et al., 2007).

Notes

This part was back translated from the corresponding amino acid sequence and optimized for expression in Escherichia coli. Codon usage has been varied for repetitive amino acids to enable DNA synthesis.

Source

Tp10 is a 21-amino acid derivative from the parent peptide transportan (originally known as galparan), which is a peptide chimera of the neuropeptide galanin and the wasp venom peptide mastoparan (Soomets et al., 2000; Langel et al., 1996).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Tp10	1,63	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K380004/1

Member of these Collections

• iGEM Parts Registry