Types | DnaRegion
|
Roles | CDS
Coding
|
Sequences | BBa_K380006_sequence (Version 1)
|
Description
Long description
Notes
An AgeI restriction site was situated at nt 340-345. As assembly standard 25 was used the restriction site was removed with site-directed mutagenesis, by changing nt 341 from C to T.
Source
Human bFGF-GST in pGEX was kindly provided by Nozomu Nishi, Kagawa University, Japan [1].
[1] Collagen-binding growth factors: production and characterization of functional fusion proteins having a collagen-binding domain