BBa_K389011

BBa_K389011 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K389011
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonas Aretz
Date created: 2010-08-09 11:00:00
Date modified: 2015-05-08 01:12:19

VirA screening device



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K389011_sequence (Version 1)

Description

This part contains:
- a mutated virG response regulator from the virA/G receptor system from Agrobacterium tumefaciens which works in Escherichia coli without an rpoA gene from A. tumefaciens
- a kanamycin resistance under the control of a vir promotor

This device is used to screen virA receptor mutants. The virA receptor is mutated in the BBa_K389010 BioBrick. The better the receptor detects a substance, the more virG is phosphorylated and the stronger the kanamycin resistance from this BioBrick is expressed. The BioBricks BBa_K389010 and BBa_K389011 have to be used together in one cell.

Notes

medium strong constitutive promotor, terminator to keep basal expression of kanamycin resistance low

Source

synthetic virG gene, vir promotor from A. tumefaciens C58, partsregistry

igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K389011/1