Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cody Tramp
Date created: 2010-07-31 11:00:00
Date modified: 2015-05-08 01:12:19
5'UTR and native RBS from psbA2 in Synechocystis sp. PCC 6803
| Types | DnaRegion |
| Roles | RBS ribosome_entry_site |
| Sequences | BBa_K390001_sequence (Version 1) |
Description
This is the 5'UTR and RBS region from gene psbA2 in Synechocystis sp. PCC 6803. It covers the entire region from the end of the promoter (as reported in Imamura S and Asayama M. 2009. Sigma Factors for Cyanobacterial Transcription. Gene Regulation and Systems Biology 3: 65-87) to immediately before the start codon of the psbA2 gene.This part can serve as a general RBS for Synechocystis sp. PCC 6803, until a library of RBS strengths in PCC 6803 is assembled. The psbA2 gene codes for the photosystem II D1 protein in this species, and therefore we assume (and plan to verify) that the RBS strength is moderate to strong, due to the high requirement for this protein in photosynthesis.
Notes
We decided to go with the full region from the promoter to the start codon. This means that, with the addition of the biobrick scar, the RBS may be located farther from the start codon then is optimal. We plan to determine optimum spacing of the RBS in Synechocystis sp. PCC 6803 in future work.Source
Genomic sequences from Synechocystis sp. PCC 6803| Sequence Annotation | Location | Component / Role(s) |
| 5' UTR with native RBS SDS core consensus native RBS | 1,49 35,38 33,42 | feature/misc sequence_feature ribosome_entry_site feature/rbs ribosome_entry_site feature/rbs |