BBa_K390004

BBa_K390004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K390004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Cody Tramp

Date created: 2010-07-31 11:00:00

Date modified: 2015-05-08 01:12:19

art+ secretion tag for Synechocystis sp. PCC 6803

Details

• Types: DnaRegion
• Roles: Tag; tag
• Sequences: BBa_K390004_sequence (Version 1)

Description

In Synechocystis sp. PCC 6803, the system of secretion is based on the charge of the N-terminus leader sequence. Positive charges on the N-terminus leader sequence contribute to secretion in a non-sequence-dependent manner (Sergeyenko TV and Los DA. 2003. Cyanobacterial leader peptides for protein secretion. FEMS Microbiology Letters 218: 351-357).

This part is an artificially designed secretion tag from the above paper. It is composed of positively charged amino acids, and was shown to successfully function as a secretion tag in Synechocystis in the above paper.

Notes

none

Source

This part is based on a sequence that was artificially designed in Sergeyenko and Los, 2003. It was created by annealing two oligionucleotides together.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start Codon	1,3	start_codon, feature/start
secretion tag - Synechocystis sp. PCC 6803	1,64	feature/tag, tag

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K390004/1

Member of these Collections

• iGEM Parts Registry