View

BBa_K391005

BBa_K391005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K391005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Phillip Chu

Date created: 2010-10-24 11:00:00

Date modified: 2015-05-08 01:12:20

Reverse primer to PCR S. aureus plasmid replicon

Back

Details

Types	DnaRegion
Roles	Primer primer
Sequences	BBa_K391005_sequence (Version 1)

Description

S. aureus plasmid replicon. Can allow a BioBrick plasmid to replicate and segregate in S. aureus. Derived from pT181. Can use either plasmids pT181 or plasmids derived from pT181 (e.g. pcN33). Add SpeI and PstI sites before using. Expect a band ~2kbp after PCR.

Notes

N/A

Source

From Charpentier, et al.. Novel Cassette-Based Shuttle Vector System for Gram-Positive Bacteria. Appl Environ Microbiol. 2004 October; 70(10): 6076???6085. Refer to pT181 gene bank information.