BBa_K395160

BBa_K395160 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K395160

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kaneta Yusuke

Date created: 2010-10-01 11:00:00

Date modified: 2015-05-08 01:12:21

RBS-CmR(no promoter and terminator)

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K395160_sequence (Version 1)

Description

We get this part ,RBS-CmR, from BBa_P1004 by PCR.
this doesn't have any promoters and terminators.

Notes

We want to create promoterless CmR on pSB6A1.
so We get this part ,RBS-CmR, from BBa_P1004 by PCR and then ligated with pSB6A1

Source

aaaatatatcatca
tgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactcca
acatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccaga
gttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatc
aagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtg
aaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctca
ggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaag
ctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttg
gacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaata
tggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS	6,9	ribosome_entry_site, feature/rbs
CmR	17,677	feature/BioBrick, engineered_region

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K395160/1

Member of these Collections

• iGEM Parts Registry