BBa_K398014

BBa_K398014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K398014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kira Schipper and Mathias Voges

Date created: 2010-10-06 11:00:00

Date modified: 2015-05-08 01:12:22

Alkane Hydroxylase System

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K398014_sequence (Version 1)

Description

The alkB gene cluster from Gordonia sp. TF6 facilitates the initial step of the degradation of C5-13 alkanes as well as that of C5-8 cycloalkanes. The cluster is formed by the genes for alkB2 (alkane 1-monooxygenase), rubA3 (rubredoxin), rubA4 (rubredoxin) and rubB (rubredoxin reductase). alkB2 is a non-haem diiron monooxygenase membrane protein. This monooxygenase oxidizes n-alkanes to the respective n-alkanols and requires three soluble electron-transfer proteins, rubredoxin (rubA3 & rubA4) and rubredoxin reductase (rubB).

Notes

Nucleotide sequence optimized for expression in E.coli

Source

Genebank

Components

Access	Instance	Definition
public	BBa_K398011	BBa_K398011
public	BBa_J61100	BBa_J61100
public	BBa_K398003	rubA4
public	BBa_J61100	BBa_J61100
public	BBa_K398004	rubB

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K398011	1,1497	BBa_K398011
BBa_J61100	1506,1517	BBa_J61100
BBa_K398003	1524,1703	rubA4
BBa_J61100	1712,1723	BBa_J61100
BBa_K398004	1730,2932	rubB

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K398014/1

Member of these Collections

• iGEM Parts Registry