402

BBa_K398402 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K398402
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hugo Federico Cueto Rojas
Date created: 2010-10-11 11:00:00
Date modified: 2015-05-08 01:12:23

Prefoldin-alpha generator



Types
DnaRegion

Roles
Translational_Unit

engineered_region

Sequences BBa_K398402_sequence (Version 1)

Description

Subunit alpha of the prefoldin from Pyrococcus horikoshii OT3. Four subunits alpha and two subunits beta create a chaperone that confers solvent tolerance to E. coli K12

Notes

Enhanced sequence for expression on E. coli K12, restriction sites for the enzymes XbaI, PstI, EcRI and SpeI are removed.

Source

The NCBI genomic sequence was in silico enhanced for iGEM purposes

Sequence Annotation Location Component / Role(s)
BBa_J23100
BBa_J61107
BBa_K398400
PhPFD-alpha
start
stop
XbaI site
1,35
44,55
62,517
62,517
62,64
515,517
36,41
feature/BioBrick engineered_region
engineered_region feature/BioBrick
engineered_region feature/BioBrick
CDS feature/cds
start_codon feature/start
stop_codon feature/stop
sequence_feature feature/misc
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K398402/1