| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K401001_sequence (Version 1)
|
Description
This construction consists of two fragments: the NM domains of the protein Sup35 and the GR526 portion (DNA-binding and transcription-activation domains) of the GR protein. The ligand-binding domain of the protein GR was eliminated, decoupling the response of the protein to the presence of glucocorticoids, and thus generating a constitutive transcription activation factor. The NM domains confers to the protein the prionic activity. The normal activity of this protein results in the activation of the genes preceded by the GRE (Glucocorticoid Response Element). When exposed to heat shock or other stress conditions, the NM domains start the prionic activity, eventually inhibiting the activation of transcription.
Notes
The NMGR526 construction can be amplified using the following primers: forward ATGTCGGATTCAAAC and reverse TCCTGCAGTGGCTTG.
Source
The NM fragment comes from the protein Sup35. The GR526 fragment comes from the protein GR. To read more about this construction, read Li and Lindquist (2000), Science, 287: 661-664.
