BBa_K407015

BBa_K407015 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K407015
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Syed Habib Tahir Bukhari, Ashwini Rahul Akkineni, Adithya Ananth, Victor Gordeev, Svea Grieb, Sarah Mansour, Mareike Roth, Charanya Sampathkumar, Lucas Schirmer, Jonathan Tam
Date created: 2010-10-25 11:00:00
Date modified: 2015-05-08 01:12:25

MBP + TEV cleavage site



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K407015_sequence (Version 1)

    Description

    The maltose binding protein (MBP) can be used as purification tag. After purification the tag can be removed using a protease specifically cutting at the TEV site (Tobacco Etch Virus site).

    Notes

    MBP increases solubility of purification products.

    Source

    genomic

    Sequence Annotation Location Component / Role(s)
    MBP
    TEV cleavage site
    		6,1146
    1158,1181
    		CDS feature/cds
    feature/tag tag

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K407015/1
     

    Attachments

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