Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Randy Pares
Date created: 2010-10-13 11:00:00
Date modified: 2015-06-15 02:56:24
ccdB minimal gene
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K414005_sequence (Version 1) |
Description
The ccdB gene is a topoisomerase II poison and will kill most cell lines. This is an improvement to the prior ccdB cell death gene (BBa_P1010) in that the size has been reduced and it is now easier to use. CcdB can be used as a selectable marker by mixing it with the desired structure and a plasmid cut with BioBrick restriction enzymes. After ligation and transformation, because ccdB is toxic to most cell lines, the only visible colonies should contain the desired structure in the desired plasmid. CcdB is toxic to most cell lines with the exception of DB3.1 cell lines (BBa_V1005).Notes
NoneSource
The ccdB gene was obtained from the U-GENT vector pK7FWG2.0| Sequence Annotation | Location | Component / Role(s) |
| ccdB gene coding region ccdB gene start codon ccdB gene stop codon Pt mutation G to A Pt mutation A to G | 330,632 330,332 632,634 103,103 321,321 | feature/cds CDS start_codon feature/start feature/stop stop_codon feature/mutation sequence_alteration sequence_alteration feature/mutation |