Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Paul Muir
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:12:26
Improved PLux/cI-OR Promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K415032_sequence (Version 1) |
Description
This part is a promoter that was designed as a correction to the anomalous BBa_R0065 BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on.Notes
SDM performed on sequence.Source
Spatiotemporal Control of Gene Expression with Pulse-Generating Networks Author(s): Subhayu Basu, Rishabh Mehreja, Stephan Thiberge, Ming-Tang Chen, Ron Weiss, Lonnie O'Neal Ingram Source: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 17 (Apr. 27, 2004), pp. 6355-6360| Sequence Annotation | Location | Component / Role(s) |
| LuxR-AHL Binding Box Pseudo-Pribnow Box Lambda Repressor OR1 Transcription Start Site | 1,21 42,47 52,68 51,51 | feature/operator operator non_covalent_binding_site feature/binding operator feature/operator start_codon feature/start |