Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2010-06-26 11:00:00
Date modified: 2015-05-08 01:12:27
RBS : pIII
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K415108_sequence (Version 1) |
Description
A copy of pIII, a surface protein that is expressed by bacteriophage M13. Since pIII allows the phage to link to E.Coli containing the F pilus, this part has applications in phage display and phage production in general.Notes
This sequence was produced via PCR, and it was determined after a graded PCR experiment that the optimal ligation temperature for pVIII was 50.5 degrees Celsius.Source
This ~1400 bp part was produced via PCR from the genome of bacteriophage M13X07. The primers read as follows:Forward: GTTTCTTCGAATTCGCGGCCGCTTCTAGAGaaagaggagaaatactagGTGAAAAAAATTATTATTCGCAATTCCTTA Reverse: [To be specified]
These primers ensured that the PCR included the standard BioBrick restriction sites.
| Sequence Annotation | Location | Component / Role(s) |
| B0034 Strong RBS pIII | 1,12 21,1295 | ribosome_entry_site feature/rbs feature/cds CDS |