Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shirley Galbiati
Date created: 2010-10-23 11:00:00
Date modified: 2015-05-08 01:12:27
K415010 + RBS : p8-GR1
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K415151_sequence (Version 1) |
Description
This part is a composite part of BBa_K415010 (PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI) with a p8-GR1 fusion appended, where p8 is the major coat protein of M13 bacteriophage and GR1 is a leucine zipper coil that can bind to corresponding zipper coil, GR2. This part is designed to produce the fusion, which can then be displayed on the phage coat when used in a helper phage system.The p8-GR1 part consists of an RBS, the p8 leader sequence, the zipper coil, an HA tag, a linker sequence, followed by a phage display optimized p8.
Notes
Linker 18 picked as one of the 15 amino acid linkers to enhance high copy protein display. opti-p8 was chosen for the same reason.Source
Sources:RBS: BBa_B0034
p8-leader sequence: First 23 amino acids of p8, exact sequenced derived from M13KE.
GR1 coil: from Wang C, Zhong P, Wang X. Adapter-directed display systems. In Vitro. 2007;17(12).
linker sequence: Linker 18 from Weiss et al. Mutational analysis of the major coat protein of M13 identifies residues that control protein display. 2000
opti-p8:from Weiss et al. 2000, derived from M13KE
The source of the p8-leader sequence is M13KE. The optimized p8 is derived from M13KE3 (see Weiss et al. Mutational analysis of the major coat protein of M13 identifies residues that control protein display. 2000).
| Access | Instance | Definition |
| public public | BBa_K415010 BBa_K415139 | BBa_K415010 BBa_K415139 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K415010 BBa_K415139 | 1,2085 2094,2528 | BBa_K415010 BBa_K415139 |